Effect of Diode Laser Irradiation and Application of Nanoparticle Herbal Endodontic Irrigation Solutions on Candida Albicans and Enterococcus Faecalis Bacteria

Document Type : Original Article


1 Restorative and Dental Materials Department, National Research Centre, Dokki, Giza 12311, Egypt

2 Laser Institute for Research and Applications LIRA, Beni-Suef University, Beni-Suef 11345, Egypt


The present study investigate whether diode laser activation of two nanoparticle herbal irrigants affect Enterococcus faecalis (E.faecalis) biofilm and Candida albicans (C. albicans) compared with photodynamic therapy (PDT) utilizing methelyene blue as photosensitizer (PS). Material and methods: sixty single rooted teeth had undergo mechanical preparation. Teeth were divided into 3 groups (20 teeth each), G1 : 20 teeth inoculated with Enterococcus faecalis half (n= 10) irrigated with nanocurcumin and the other half irrigated with nanoneem both activated by diode laser, G2: 20 teeth inoculated with Candida albicans irrigated similar to group 1 and G3: 10 teeth inoculated with E.faecalis and 10 with Candida albicans undergo PDT using methylene blue as PS. Results: For E.faecalis no statistically significant difference neither between nanoneem and nanocurcumin (p>0.05) nor between nanocurcumin and PDT (p>0.05). While there was a statistically significant difference between nanoneem and PDT (p<0.001). While for C. albicans no statistically significant difference between nanocurcumin and PDT (p>0.05). While the differences were statistically highly significant between nanoneem and PDT (p=0.002) and between nanoneem and nanocurcumin (p<0.001). Conclusion: nanoneem and nanocurcumin irrigants activated by laser have an effect on root canal microorganisms and could be used as adjunctive endodontic irrigant.


Main Subjects

Volume 67, Issue 13 - Serial Number 13
In Loving Memory of Late Professor Doctor ””Mohamed Refaat Hussein Mahran””
December 2024
Pages 331-340
  • Receive Date: 23 December 2023
  • Revise Date: 06 February 2024
  • Accept Date: 07 February 2024