Spectrophotometric Determination of Metronidazole in Pharmaceutical Preparations and in Human Blood samples

Document Type : Original Article

Authors

1 Collage of pharmacy/University of Mosul

2 Department of chemistry/college of science/university of Mosul

3 College of Education for Pure Sciences University of Mosul

Abstract

This research investigates a sensitive quantitative estimation of metronidazole (MZOL) in each of its pharmaceutical preparations and its level in human blood with one color reaction and one simple spectrophotometric technique. Metronidazole the antibiotic and antiprotozoal medication is determined by the reduction reaction of MZOL to 2-(2-Methyl-5-amino-1H-imidazole-1-yl) ethanol, followed by coupling with diazotized p-amino benzophenone (PABPh) reagent. The produced color complex is measured at 431 nm. The proposed method is successfully applied for the determination of metronidazole in different dosage forms (tablet, suspension, and intravenous injection) with high precision (RSD% from ±0.011 to ±2.3). as well as it applied for the determination of metronidazole in human blood samples of healthy voluntaries easily and accurately after 4, 6, 12, and 24 hours of oral administration with relative standard deviation not more than ±1.922 which provide the ability for therapeutic monitoring follow up of metronidazole in blood.

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History

  • Receive Date: 25 December 2021
  • Revise Date: 08 January 2022
  • Accept Date: 13 January 2022

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