Green Synthesis of Zinc oxide Nanocomposite Using Fusarium oxysporum and Evaluation of the Anticancer Effect on Hepatocellular Carcinoma

Rashwan, Doaa Sayed; Abd El Hamed, May Mohamed; El-deen, Mohamed Diaa; Afify, Mei Mohamed; Mohamed, Mona Hassan; Mohamed, Abd El Razik Farrag; Nagy, Rana Assem; A. Elhakim, Heba Kamal

doi:10.21608/ejchem.2021.91841.4361

Green Synthesis of Zinc oxide Nanocomposite Using Fusarium oxysporum and Evaluation of the Anticancer Effect on Hepatocellular Carcinoma

Document Type : Original Article

Authors

¹ 1 Biochemistry Division, Faculty of Science, Cairo University, Giza, 12613, Egypt 2 Faculty of Medicine, Galala University, Suez, 43511, Egypt

² Spectroscopy Department, National Research Centre, Dokki, Giza, 12622, Egypt

³ Department of Biochemistry, Genetic Engineering and Biotechnology Research Division, National Research Centre, Dokki, Giza, 12622, Egypt

⁴ Department of Chemistry, Faculty of Science, Cairo University, Giza, 12613, Egypt

⁵ Professor of Histology and Histochemistry, Pathology Department, Medical Research Division, National Research Centre, Dokki, Giza, 12622, Egypt

⁶ Biochemistry Division, Faculty of Science, Cairo University, Giza, 12613, Egypt

⁷ Biochemistry, faculty of Science, Cairo university, Giza, Egypt

10.21608/ejchem.2021.91841.4361

Abstract

Nanoparticles can be synthesized by chemical, physical and biological system methods. In this work, Superparamagnetic Iron Oxide@Silver nanoparticles (SPION@Ag NPs) were modified by zinc oxide nanoparticles (ZnO NPs). SPION@Ag core shell NPs were synthesized by the biological method using Fusarium oxysporum fungus and coated with ZnO NPs. This method is eco-friendly and low cost. Characterization techniques such as UV, FTIR, HR-TEM, HR-SEM, XRD and zeta potential were used. The most common type of liver cancer is hepatocellular carcinoma (HCC). Hepatocellular carcinoma was induced by a single dose of diethyl nitrosamine (DEN) 60 mg/Kg b.wt. and was followed after two days with carbon tetra chloride (CCl4) diluted with paraffin oil (50% v/v, 2 ml/Kg b.wt.) twice a week for one month. A histopathology examination was done after scarifying. Clear changes were shown in the comparison between the HCC group treated with ZnO@SPION@Ag nanocomposite and the positive control group. Liver function tests showed a highly significant decrease after using the nanocomposite for HCC treatment. Our study aim is to evaluate the therapeutic anticancer effect of ZnO@SPION@Ag nanocomposite on hepatocellular carcinoma in male albino rats, which was synthesized by a new method called green chemistry or green synthesis.

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