LC-ESI-MS/MS Analysis of Bioactive Metabolites from Streptomyces rochei with Antimicrobial, Antioxidant, and Cytotoxic Properties

Document Type : Original Article

Authors

1 Microbiology Department, Faculty of Agriculture, Cairo University, 12613 Giza, Egypt

2 Central Lab. of Organic Agriculture, A.R.C, Cairo, Egypt

Abstract

Actinomyces, particularly Streptomyces, have a major impact on protecting plant roots by inhibiting the growth of fungal species based on their capacity to release antifungal antibiotics. Herein, FT-IR spectra for Streptomyces rochei extract revealed the varied patterns of functional group absorption of the bioactive compounds (BACs). Furthermore, using LC-ESI-MS/MS analysis, 21 novel diverse secondary metabolite compounds from S. rochei extract were found: nine flavonoids, one flavonoid derivative, one phytohormone, eight phenolic acids, one benzoic acid esters and one triterpenoid. Likewise, a DPPH radical scavenging capacity experiment was conducted to assess the antioxidant properties of four different concentrations (50, 100, 150 and 200 µg/ml) of S. rochei extract. In addition, BACs from S. rochei exhibited significant antibacterial activity against dangerous human pathogens Bacillus cereus, Staphylococcus aureus and gram-negative bacteria Salmonella typhimurium. Also, antimycotic activity against Candida albicans was measured. S. rochei extract showed a moderate level of cytotoxicity against lung cancer cell lines (A-549) with low cytotoxic effects on lung normal cell lines (WI-38). The biological activities of streptomyces extract might be attributed to the presence of a variety of metabolites, and hence, S. rochei can be used as an excellent source of bioactive metabolites.

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Egyptian Journal of Chemistry
Volume 67, Issue 13 - Serial Number 13
In Loving Memory of Late Professor Doctor ””Mohamed Refaat Hussein Mahran””
December 2024
Pages 1947-1956

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History

  • Receive Date: 21 September 2024
  • Revise Date: 08 October 2024
  • Accept Date: 17 October 2024

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