Utilizing Biowaste-Derived Chemicals in Designing, Preparing, and Evaluating the Biopesticidal Activity of A New Formulation For Controlling Root Rot and Root Knot Infection in Green Beans

Document Type : Original Article


1 Green chemistry department, National Research Centre

2 Green Chemistry department, National research centre

3 Plant Pathology Department, National Research Centre, Cairo, Egypt.


The current study investigates the use of alkaline black liquor (ABL), a byproduct of the rice straw solar pulping process, to create a bioactive formulation that can inhibit the growth of soilborne plant pathogens. ABL was first treated to precipitate lignin, silica, and fatty acids as a calcium complex (Ca-LSF). The resulting highly alkaline effluent after chemical analyses was used to hydrolyze chicken feather waste to its amino acid constituents. The resulting new protein hydrolysate was chemically analyzed and then added to the Ca-LSF complex, affording a novel formulation with slow-release nitrogen, carbon, and bioactive phenolic sources to be evaluated against soilborne pathogenic fungi Fusarium oxysporum, Fusarium solani, Rhizoctonia solani, and Sclerotium rolfsii, as well as the nematode Meloidogyne incognita, the causal organisms of wilt, root rot, and root knot diseases. The prepared formulation showed a strong inhibitory effect against R. solani and affected the viability of M. incognita. This effect increased with increasing concentration, up to 4%, which completely inhibited fungal growth. The presented safe, cost-effective, and eco-friendly formulation could be used to control a wide range of soilborne plant pathogens; moreover, it presents a complete recycling of two hazardous biowastes via zero-waste processes.


Main Subjects

Volume 67, Issue 13 - Serial Number 13
In Loving Memory of Late Professor Doctor ””Mohamed Refaat Hussein Mahran””
December 2024
Pages 511-518
  • Receive Date: 25 December 2023
  • Revise Date: 29 January 2024
  • Accept Date: 05 February 2024