Facile One-Pot Strategy for Radiocoplexation of [99mtc]-Nitrido-Nebracetam for Brain Imaging: Biological Evaluation, Optimized Chromatographic Separation and Labeling Conditions

Document Type : Original Article

Authors

1 Labeled Compounds Department, Hot Labs Center, Egyptian Atomic Energy Authority, P.O. Box 13759, Cairo, Egypt.

2 Pharmaceutical Chemistry Department, Faculty of Pharmacy, Ahram Canadian University, Giza, 12578 Egypt

3 Food Toxicology and Contaminants Department, National Research Centre, Dokki, Cairo, Egypt.

Abstract

Glutamatergic α-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid receptors (AMPARs) are central to regulating excitatory synaptic transmission in the central nervous system (CNS) Therefore, the aim of this research is to study the biodistribution to demonstrate the concentration of [99 mTc]-nitrido-nebracetam in the target organ (brain) using healthy groups of Swiss albino mice. The radiotracer [99mTc]-nitrido-nebracetam ([99mTc]-N-Neb) complex was labelled using the core [99mTc≡N] 2+.Many factors have been studied to optimize high radiochemical yields (>97.5).Quality control studies were made perfectly. A process of biodistribution indicated that this complex, [99mTc]-N-tamsulosin, was washed out through the urinary pathway within 3 hours after injection. In addition, the brain uptake of this complex was 11.88% after 30 min post-injection as a novel probe for (AMPA) receptor targeting. Hence, the radiotracer of [99mTc]-N-Neb could be used as a radiotracer for brain imaging through the AMPA receptor.

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Egyptian Journal of Chemistry
Volume 67, Issue 13 - Serial Number 13
In Loving Memory of Late Professor Doctor ””Mohamed Refaat Hussein Mahran””
December 2024
Pages 471-480

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History

  • Receive Date: 24 December 2023
  • Revise Date: 15 January 2024
  • Accept Date: 24 January 2024

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