In vitro anticancer and antimicrobial activities of copper-zinc superoxide dismutase purified from Cellana rota snail

Habib, Mohamed R.; Masoud, Hassan M.; Helmy, Mohamed S.; Hamed, Ahmed A.; Gad El-Karim, Rasha M.; Ali, Rasha E.M.; Zayed, Khaled M.; Ibrahim, Mahmoud A.

doi:10.21608/ejchem.2023.200467.7736

In vitro anticancer and antimicrobial activities of copper-zinc superoxide dismutase purified from Cellana rota snail

Document Type : Original Article

Authors

¹ Medical Malacology Dept., Theodor Bilharz Research Institute, 1 Corniche El Nile St., Warrak El-Haddar, Imbaba, Giza 12411, Egypt

² Molecular Biology Dept., National Research Center, 33 El-Buhouth St., Dokki, Giza 12622, Egypt

³ Proteome Research Lab., Central Laboratories Network and Centers of Excellence, National Research Center, 33 El-Buhouth St., Dokki, Giza 12622, Egypt

⁴ Microbial Chemistry Dept., National Research Center, 33 El-Buhouth St., Dokki, Giza 12622, Egypt

10.21608/ejchem.2023.200467.7736

Abstract

The antioxidant, antimicrobial and anticancer activities of molluscan gastropods were elucidated in numerous previous studies. One of the most important antioxidant enzymes is superoxide dismutase (SOD) was homogeneously purified and characterized from Cellana rota snail crude extract. The purification of C. rota SOD was carried out through chromatographical separation on anion exchanger and size-exclusion resins. The specific activity of the purified SOD was 520.7 units mg-1 protein, representing 6.6 folds over the crude extract. The purified SOD was found 180-kDa monomeric as detected by gel filtration and SDS PAGE. It was greatly enhanced with CuCl2 and ZnCl2 and strongly inhibited with KCN and H2O2, proposing it as a Cu/Zn-SOD enzyme that termed Cellana rota copper-zinc superoxide dismutase (CrCu/Zn-SOD). The antimicrobial screening of CrCu/Zn-SOD indicated its antibacterial capacity against E. coli, S. typhi and S. aureus with MIC value of 0.78 µg/mL for the three pathogens. Moreover, it showed a MIC value of 1.56 µg/mL against the fungus A. niger compared to 5 µg/mL nystatin. CrCu/Zn-SOD showed potent in vitro toxicity towards adenocarcinomic human alveolar basal epithelial cells A549, human colorectal carcinoma Caco2 and human hepatocellular carcinoma HepG2 cancer cells with 2.451, 3.933 and 10.5 μg/mL IC50 values respectively. These preceding results suggest that CrCu/Zn-SOD from C. rota snail may have the potential to serve as a substrate for future antimicrobial and anticancer drug development.

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