Document Type : Original Article
Authors
1
Environmental Biotechnology Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), City of Scientific Research and Technological Applications (SRTA-CITY), Borg El-Arab New City, Alexandria 21934, Egypt.
2
Chemistry Department, College of Science, Jouf University, P.O. Box: 2014, Sakaka, Saudi Arabia. - Department of Chemistry, Faculty of Science (Girls), Al-Azhar University, Yousef Abbas Str., Nasr City, Cairo, Egypt
3
Department of Chemistry, Faculty of Science (Girls), Al-Azhar University, Yousef Abbas Str., Nasr City, Cairo, Egypt.
4
Polymer Materials Research Department, Advanced Technology and New Materials Research Institute, City of Scientific Research and Technological Applications (SRTA-CITY), Borg El-Arab New City, Alexandria 21934, Egypt.
5
Environment and Natural Materials Research Institute (ENMRI), City of Scientific Research and Technological Applications, (SRTA-CITY), Borg El-Arab New City, Alexandria 21934, Egypt.
6
Chemistry Department, College of Science, Jouf University, P.O. Box: 2014, Sakaka, Saudi Arabia.
7
Food Technology Research Institute, Agriculture Research Center, Giza, Egypt
Abstract
Rice waste as a starchy food waste that is discharged daily into the environment in huge amounts has been used as a valuable source for the production of bioethanol. The tested parameters investigated that phosphoric acid as a chemical representative combined with autoclaving as a physical representative are the most potent conditions for the liberation of glucose units from the starch molecules among other tested conditions. The optimization process revealed that the maximum production of simple sugars of 12.39 mg/ml from rice waste can be obtained through the pre-treatment of 5% rice waste with 20% H3PO4 and autoclaving followed by the amylase hydrolysis. These liberated glucose units have been successfully fermented into 5.2 mg/ml bioethanol using a yeast strain (Pichia nakasei with the accession number OK092294). The separation of the produced bioethanol from other media components was performed via amicon cell ultrafiltration system integrated with polymeric membrane. The membrane was prepared from Polyvinyl chloride/Polymethylmethacrylate/ Graphite [PVC/PMMA/G] with different thicknesses 400, 500 and 600 µm. For membrane characterization, Fourier transform infrared (FT-IR), scanning electron microscope (SEM), tensile strength, water contact angle, and water and ethanol uptakes have been used. Among the tested membranes thicknesses, the obtained results showed that the best membrane thickness is 500 µm which recoded 939.526 (mg/m2.h) and 10.557 for flux of permeate and separation factor, respectively.
Keywords
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