Detection of Lysozyme and Antioxidant Enzymes in Normal and Immunized Haemolymph of the Silkworm, Bombyx mori During theImmature Stage

Shahin, Rima Rabiea; El-Agamy, Elsayed

doi:10.21608/ejchem.2021.111076.5058

Detection of Lysozyme and Antioxidant Enzymes in Normal and Immunized Haemolymph of the Silkworm, Bombyx mori During theImmature Stage

Document Type : Review Articles

Authors

¹ Department of Applied Entomology and Zoology, Faculty of Agriculture, Alexandria University, Egypt.

² Alexandria University Faculty of Agriculture · Department of Dairy Science Ph.D. Immunovirology

10.21608/ejchem.2021.111076.5058

Abstract

The activity of lysozyme (EC 3.2.1.17), as one of the immune system components of silkworm Bombyx mori L., was determined in the cell-free hemolymph of fifth larval instar by the lyso- plate assay using Micrococcus lysodeikticus. The lysozyme activity of the beginning newly larvae was 39 μg/ml, then increased to 248 μg/ml of hemolymph in mature larvae. The injection of Staphylococcus aureus and Escherichia coli into larvae resulted in an increase in lysozyme activity within 24 hours to 274 and 328 μg/ml, respectively. Antioxidant enzymes, including catalase (EC 1.11.1.6), peroxidase (EC 1.11.1.7), and ascorbate peroxidase (EC 1.11.1.11) have been detected also in hemolymph using isoenzyme native-polyacrylamide gel electrophoresis. The activities of catalase and ascorbate peroxidase isoenzymes were increased with time progress, especially at the wonder stage while, a slight increase in peroxidase activity was observed.

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