Document Type : Original Article
Authors
1
Chemistry of Medicinal Plants Department, Division of Pharmaceutical and Drug Industries, National Research Centre, Buhouth St. (Former El Tahrir St.), 12622, Dokki, Giza, Egypt.
2
department of pharmacognosy, faculty of pharmacy, Cairo university
3
Pharmacognosy Department, Faculty of Pharmacy. Cairo University
4
aChemistry of Medicinal Plants Department, Division of Pharmaceutical and Drug Industries, National Research Centre, Buhouth St. (Former El Tahrir St.), 12622, Dokki, Giza, Egypt.
5
Plant Biotechnology Department, Genetic Engineering and Biotechnology Division, National Research Centre (NRC), Dokki, Cairo, Egypt
Abstract
Valeriana officinalis L is the most cost-effective plant in Europe. Due to its cardiovascular, anxiolytic, sedative, and anticonvulsant properties, the root of Valeriana is extensively employed in herbal therapies. Seeds of Valeriana were germinated in vitro by soaking for 24 hours in 150 ppm Gibberellic acid (GA3) further, cultivated in half MS (Murashige and Skoog salts medium with vitamins)medium. Treatment of MS media with 5 ppm of Benzyl amino purine (BA) and 2,4-Dichlorophenoxy acetic acid (2,4-D) increased fresh and dry weights of calli produced from leaf explants. However, supplemented MS media with 3 ppm BA and 1 ppm 2,4-D enhanced the efficiency rate of regeneration. On the other hand, augmentation of MS-medium with 0.1 ppm Indole-3-butyric acid(IBA), achieved of rooting process, while acclimatization was accomplished by using peat moss:vermiculite (1:1). Valerenic acid and valtrate were analyzed qualitatively and quantitatively by HPLC in mother plant, callus, regenerated shoots, roots, and Dormival medication which used as a control. The findings showed that regenerated shoots had the greatest concentration of valerenic acid across various extracts (3.80±0.82mg/g DW), whereas in vitro-derived roots from leaf explants contained the highest concentration of valtrate (3.82±0.23mg/g DW).
Keywords
Main Subjects
)
View on SCiNiTO