The potential Bio-indicator species such as Pomacea canaliculata (Family: Ampullariidae) resources could be utilized as a biological model for monitoring the anthropogenic pollution in freshwater environments. In the present study, the P. canaliculata biological responses to silver nanoparticles (synthesized via the green method) were evaluated. The similarity values among the estimated snail samples were calculated based on the analysis of morphometric (shell morphology) and molecular variations (via Inter simple sequence repeats). The normality of morphometric results was checked before scouting quantitative shell measurements. The results did not differ significantly from that which is normally distributed. The snail individuals were reared for certain time intervals under silver nanoparticles’ stresses. Saline soluble protein subunits’ separations and some isozyme systems (Glucose6 phosphate dehydrogenase, Superoxide dismutase, Esterase, and Malate dehydrogenase) were applied to detect biochemical tags due to the treatment effects. No differences were detected among the evaluated samples concerning the Glucose 6 phosphate dehydrogenase separations. The separations of the other systems exhibited some tags between the control and the treated individuals. The numbers and/or the intensities (as sensitive biochemical probes of differential gene expression in the snails ‘foot muscle samples) of isozyme bands were affected by the silver Nanoparticles treatment under the experiment condition. More informative markers could be detected and evaluated for monitoring aquatic ecological health at water pollution level in the future Eco-Toxicological studies.
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