Bioengineering of Zinc Oxide Nanoparticles As Therapeutics for Immunomodulatory and Antimicrobial Activities

Document Type : Original Article


1 Prof. of medical microbiology,Botany and Microbiology Department, Faculty Science Al-Azhar University

2 2Applied-Science & Robotics Laboratory for Applied-Mechatronics (ARAtronics Lab.); Mechatronics Engineering Department, German University in Cairo, New Cairo 11835, Egypt.

3 Department of Botany and Microbiology, Faculty of Science, Al-Azhar University,11884 Nasr City, Cairo, Egypt

4 Ph.D. Affiliation: Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Cairo 11884, Egypt.


In the present work, eco-friendly biosynthesis of zinc oxide nanoparticles (ZnONPs) and their immunostimulatory and antimicrobial activity were investigated. Streptomyces rochei, the ZnONPs manufacturer, were isolated and identified. The morphology and characterization of ZnONPs using UV-Vis, DLS, TEM, and XRD, were investigated. The results showed that the spherical shape of ZnONPs was with a mean diameter of about 36nm. The viability of the HFB-4 and Vero cells was decreased to 42 and 46% respectively after treatment with a dose of 16µl/ml of ZnONPs. ZnONPs showed a significant immunostimulatory effect with 137.2 ± 0.18% and increased CD4+ and CD8+ lymphocytes level with 85.2 ± 0.07% and 25.3 ± 0.03%, respectively. Also, they exhibited an antimicrobial activity against both Gram-negative, positive bacteria and fungi with inhibition zone ranging from 16.0 to 28.0 mm and MIC ranging from 8.67 to 125.0 µg/ml. This study highlights that ZnONPs strongly stimulate immune system and have antimicrobial activities.


Main Subjects

Volume 65, Issue 131 - Serial Number 13
Special Issue: Chemistry and Global Challenges (Part A)
December 2022
Pages 1473-1486
  • Receive Date: 28 April 2022
  • Revise Date: 13 June 2022
  • Accept Date: 19 June 2022