Effect of Ginkgo biloba leaf extract in combination with vitamin C, E and D on Aluminum Chloride induced Alzheimer in rats.

Document Type : Original Article


1 Pharmacognosy Department, National Research Centre

2 Biotechnology Department, Faculty of Science, Cairo University

3 Biotechnology Department , Faculty of Science, Cairo University.

4 National research center


Alzheimer’s disease(AD), is a neurodegenerative ailment and the most prevalent cause of dementia. The commonly used synthetic drugs provide temporary and incomplete symptomatic treatment accompanied with severe side effects. The present study aimed at evaluating the therapeutic potential of Ginko Biloba, and/ or Vitamin D, E, C on aluminum chloride (AlCl3)-induced neurotoxicity in rats.

AD was induced in rats by oral administration of AlCl3 (17 mg/kg BW) for 1 month. Rats were treated with Ginko Biloba methanolic leaf extract (400 mg/kg.BW/day), vitamin D3 (0.0125 mg/kg.BW/day), E(2.5mg/kg.BW/day), Vitamin C (50mg/kg.BW/day) and their combinations, respectively, for 15 days. Behavioral assessment was carried out using Y-maze test. The activity of acetylcholinesterase, Dopamine, Serotonin and Nor-Epinephrine; in addition, histopathological examination of the isolated Hippocampal brain tissues were done. It was observed that administration of Ginko biloba in combination with vitamin C attenuated memory impairment and improved cholinergic and dopaminergic dysfunction. Also, significant improvement in histopathological alterations and hippocampal morphology was noticed. It could be concluded that Ginko biloba and /or vitamin C exhibited significant improvement in memory loss and neurotoxicity and could be an effective treatment against cognitive decline in AD patients.


Main Subjects

Volume 65, Issue 131 - Serial Number 13
Special Issue: Chemistry and Global Challenges (Part A)
December 2022
Pages 827-841
  • Receive Date: 13 April 2022
  • Revise Date: 06 June 2022
  • Accept Date: 09 June 2022