Gas Chromatography-Mass Spectrometry Analysis of Phytocomponents Present in Pimpinella anisum L. Callus Cultures as Affected by Yeast and Phenylalanine Application

Document Type : Original Article


1 National Research Center

2 Department of Pathology, National Cancer Institute, Cairo University, Cairo, Egypt

3 Plant Biotechnology, Biotechnology Research Institute, National Research Center


Long times ago, anise (Pimpinella anisum L.) plant, which belonging to umbelliferae family, was used in Egyptian folk medicine. In the current study, the effects of yeast extract as an elicitor and phenylalanine as a precursor on the accumulation of bioactive constituents in anise callus cultures were documented by gas chromatography-mass spectrometry (GC-MS) techniques. It is revealed that P. anisum callus extracts contain different biologically active compounds with antioxidant, antimicrobial and anticancer potential. Cytotoxicity of anise callus extracts was studied to illuminate the vital assignment of many active constituents in diminution of the breast cancer growth and also their antitumor activity.


Main Subjects

Volume 65, Issue 131 - Serial Number 13
Special Issue: Chemistry and Global Challenges (Part A)
December 2022
Pages 667-675
  • Receive Date: 27 March 2022
  • Revise Date: 21 May 2022
  • Accept Date: 25 May 2022