Sugarcane bagasse wastes represent untraditional pillars for antifungal silica-based nanoparticle and ß-glucosidase production

Document Type : Original Article

Authors

1 Microbiology Dept., Soil, Water and Environment Research Institute, Agricultural research Centre

2 Biotechnology Department, Faculty of Graduate Studies and Environmental Researches, Ain Shams University, Cairo, Egypt

3 Microbiology Dept., Faculty of Agriculture, Cairo University, Giza, Egypt

4 Department of Microbiology, Faculty of Agriculture, Cairo University, Giza, Egypt

Abstract

As a natural raw waste of agricultural origin, sugarcane bagasse has several potential future applications upon converting into promising products. Both alkaline-treated solution and cellulose pulp were used for preparation of antifungal silica-based nanoparticles (Si-NPs) beside glucose and production. Adopting the agar-disc diffusion technique, nanosilica successfully exhibited in vitro antifungal activity towards the pathogen Fusarium solani, inhibition zones of 0.4-0.9 mm dia. were measured. In a pot experiment, increasing levels of NPK fertilization regimes and Si-NPs were examined for protecting potatoes from the fungal attack. The highest rates of both amendments supported sufficient tuber yield and vegetative growth, plants heavily dressed hosted 9-25 tubers plant-1 with fresh weights of 159.9-550.6 g plant-1. The pathogenicity of F. solani towards the vegetable plant significantly diminished in presence of NPK and Si-NPs. Tuber biomasses positively correlated with NPK doses (r, 0.918) and Si-NP rates (r, 1.00), except the low level of 25 % that failed to promoted growth and yield even in presence of full NPK regime. A. niger succeeded to convert cellulose pulp into reducing sugars and ß. glucosidase. The enzyme production significantly optimized after 12 hr. incubation at 200 rpm particularly in culture medium amended with 3 % corn speed liquor. Interestingly, adding 5 ml drop-wise distilled water to β. glucosidase production medium seemed necessary to avoid the feedback inhibition of the enzyme.

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Volume 65, Issue 13 - Serial Number 13
Special Issue: Chemistry and Global Challenges
December 2022
  • Receive Date: 10 April 2022
  • Revise Date: 10 May 2022
  • Accept Date: 10 May 2022
  • First Publish Date: 10 May 2022