Comparative characterization of carob pulp and seeds extracts: HPLC, antimicrobial, anti-inflammatory, and cytotoxic studies

Document Type : Original Article

Authors

1 faculty of pharmacy October 6 university

2 Pharmacology and Toxicology Department, Faculty of Pharmacy, October 6 University, Giza, Egypt.

3 Pharmacology Department, Medical Research and Clinical Studies Institute, National Research Centre, Dokki, Giza, Egypt

4 Microbiology and Immunology Department, Faculty of Pharmacy, October 6 University, Giza, Egypt

5 Pharmacognosy Department, Faculty of Pharmacy, October 6 University, Giza, Egypt.

Abstract

HPLC profile, antimicrobial, cytotoxic, and anti-inflammatory activities of carob (Ceratonia siliqua L.) pulp and seeds extracts were investigated. Gallic acid was the main identified phenolic. Ellagic acid, rutin, quercetin, and kaempferol were also tentatively identified. Carob seeds extract exhibited antimicrobial activity against Pseudomonas aeruginosa (6.25 and 25 mg mL-1 for minimum inhibitory concentration and minimum bactericidal concentration: respectively). Carob extracts showed a weak dose-response inhibition of the lipopolysaccharide induced nitric oxide release in RAW macrophages. Carob extracts even in the high dose did not inhibit the cell growth either against MCF-7 or HT-29 cell lines. Carob pods could be used as a functional food or food additive based on these findings.

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Volume 65, Issue 10
October 2022
Pages 279-284
  • Receive Date: 16 January 2022
  • Revise Date: 07 February 2022
  • Accept Date: 22 February 2022
  • First Publish Date: 22 February 2022