Hyaluronic acid /carboxymethyl chitosan embedded gold nanoparticles modulate high fructose diet induced diabetes changes in rats

Document Type : Original Article

Authors

1 Department of nutritional chemistry and metabolism, National Nutrition Institute, 11562Cairo, Egypt.

2 Chemistry Department, Faculty of Science(Girls), Al-Azhar University, Nasr City, Cairo.

3 Department of nutritional chemistry and metabolism, National Nutrition Institute

4 1- 11884-Nasr City, Al-Azhar University, Faculty of Science, Botany and Microbiology Department 2- University of Jeddah, College of Sciences and Arts at Khulais, Department of Biology

5 Textile Research Division, National Research Centre, Cairo, Egypt

Abstract

Abstract
In this current work, hyaluronic acid (HA)/carboxymethyl chitosan (CMCTs) blend, embedded with definite amount of gold nanoparticles “AuNPs”, are utilized to mitigate diabetiec parameters in experimental model of diabetes in rats. AuNPs was produced thru Microwave radiation technique, followed by characterization using state of art anlysis; UV and TEM. Afterward, 50" male albino rats" were divided into two main groups: Group one "n=10 rats" fed on the healthy diet (-ve control). The 2nd group fed on the high fructose diet (HFD) for 4 weeks to induce diabetes. To this end, the 2nd group, is further divided into four sub-groups. The 1st one (G2) received only HFD (+ve control), 2nd,3rd and 4th subgroups (G3, G4 and G5) received orally; mixture of both HA and CMCTS in different ratios (1:3; 1:1; 3:1 respectively) embedded all with AuNPs (0.01M), in a dose of 2 mg/kg body weight /day for 4 weeks. In addition, the effect of polymers blends with AuNPs on Streptococcus faecalis, Escherichia coli and Aspergillus fumigatus was also evaluated. All treated groups (G3, G4 ,G5) decreased diabetic biomarkers Fasting blood sugar (FBS), fasting insulin, glycosylated hemoglobin (HbA1C), "Homeostasis model assessment of insulin resistance (HOMA-IR)" index; "homeostasis model assessment of β-cell function (HOMA-β) "combared with the +ve control. Moreover, the mixture of HA/CMCTS (1:3) adorned AuNPs was more potent than the other used ratios from the polymer combination, in attenuation of diabetic intricacy, however, the antimicrobial activity for all mixture was effective but almost the same. Furthermore, all treated subgroups disclosed normal histopathological pancreatic cell structures.

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  • Receive Date: 25 December 2021
  • Revise Date: 30 January 2022
  • Accept Date: 05 February 2022
  • First Publish Date: 05 February 2022