In vitro cytotoxic activity and antioxidant potential of different extracts from Pittosporum eugenioides

Hamed, Manal M.; Refahy, Laila A.; Shafei, Azza A.; Abd El Aziz, Eman G.I.

doi:10.21608/ejchem.2022.113405.5189

In vitro cytotoxic activity and antioxidant potential of different extracts from Pittosporum eugenioides

Document Type : Original Article

Authors

¹ Medicinal Chemistry Department, Theodor Bilharz Research Institute, Giza, Egypt

² Faculty of Pharmacy, Al-Azhar University, Cairo, Egypt

³ Sheben Elkom Hospital, Menofiya, Egypt

10.21608/ejchem.2022.113405.5189

Abstract

Extracts of different polarity from Pittosporum eugenioides leaves, were investigated for their antioxidant and cytotoxic activity. Two different bioassays were used, namely scavenging of the diphenylpicrylhydrazyl (DPPH) radical and total antioxidant capacity (TAC). The butanol extract of P. eugenioides leaves record the strongest free radical scavenging one among the tested extracts with SC50 value of 26.38 μg/ml. The total flavonoid content (TFC) of the plant extracts was ranged from 212.95 to 540.85mg RE / g dry extract and the total phenolic content (TPC) of the tested extracts was ranged from 292.12 to 934.25 mg gallic acid equivalent (GAE)/g dry extract. Positive correlations were found between total phenolic content in the extracts and antioxidant activity, (R2 = 0.9998, DPPH and R2 =0.9302, TAC). Considering LC50, the most promising cytotoxic fraction against brine shrimp (Artemia salina L.) was the n-butanol extract (34μg/ml) followed by 85% methanol extract (45μg/ml). In conclusion, P. eugenioides exhibit great potential for antioxidant and cytotoxic activity and may provide good source of natural antioxidant and thus inhibit unwanted oxidation processes, so it may be useful for medicinal functions.

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