A Comparative study of Chemical Compositions, Antimicrobial and Antiviral Activities of Essential Oils for Citrus medica var. sarcodactylis Swingle Fruits and Leaves along with Limonia acidissima L. Leaves.

El Hawary, Seham Salah Eldin; Hashim, Fatma A.; Ibrahim, Nabaweya A.; Matloub, Azza A. E.M.; ElSayed, Abeer M; Farid, Mohamed A; Shata, Hoda M. A. H.; Kutkat, Omnia; El-Abd, Eman A.W.; Ali, Mohamed

doi:10.21608/ejchem.2021.108413.4958

A Comparative study of Chemical Compositions, Antimicrobial and Antiviral Activities of Essential Oils for Citrus medica var. sarcodactylis Swingle Fruits and Leaves along with Limonia acidissima L. Leaves.

Document Type : Original Article

Authors

¹ Pharmacognosy Department, Faculty of Pharmacy, Cairo University

² Department of Pharmacognosy, National Research Centre; 33-Elbohouth St, (Former El-Tahrir St.), Dokki –Giza-Egypt. P.O.12622, ID: 60014618.

³ Abeer Kasr El Aini street Cairo

⁴ Natural and Microbial Products Department, National Research center

⁵ Microbial Chemistry Department, National Research Centre, Dokki, Giza-Egypt. P.O.12622

⁶ National Research Centre, Water pollution Department, Center of Scientific Excellence for Influenza Viruses, 33 El buhouth Street, P.O. Box 12622, Giza, Egypt.

10.21608/ejchem.2021.108413.4958

Abstract

The chemical composition of fruits and leaves derived oil of Citrus medica var. sarcodactylis Swingle (CMF&CML) and leaves of Limonia acidissima L. (LAL), Rutaceae were analyzed by gas chromatography-mass spectroscopy (GC-MS). Twenty-one components accounting for (98.51%) were identified in CMF oil. While in leaves oil, fourteen components were identified accounting for (92.69%) of the total oil content and the major component is limonene for both CMF and CML oil representing 60.31% and 32.11% respectively. Oil derived of LAL prevailed eight components were representing (97.90%) of its total oil and the major identified component is carvacrol (71.15%) Oils derived of fruits, leaves of C. medica and leaves of L. acidissima were investigated for their antimicrobial and antifungal activities. Fruits-derived oil exhibited potent antimicrobial activity than the leaves of both plants. Antifungal activity was moderate for fruits essential oil against Aspergillus niger. Oil derived of C. medica var. Sarcodactylis and L. acidissima leaves at concentration 0.5 µg/µl showed comparable antiviral activity against avian influenza A H5N1 virus. It was the first comparative study for essential oils of C. medica var. Sarcodactylis fruits, leaves, and L. acidissima L. leaves and their antiviral activity against H5N1 virus.

Keywords