Document Type : Original Article
Authors
1
Physics Department, Faculty of Science, Ibb University,Ibb, Yemen
2
Chemistry Department, Faculty of Science, Ain Shams University, Abbassia, 11566, Cairo, Egypt
3
Chemistry Department, Faculty of Science, Ain Shams University
Abstract
There is great research interest in synthesizing nanomaterials using environmentally friendly methods due to the many applications of these materials in several fields. In this paper, a facile and green chemistry method was used to synthesize silver nanoparticles (AgNPs) using the Egyptian propolis as reducing and capping agent. Different parameters such as aqueous propolis extract quantity, pH, reaction time, and temperature were investigated to get the optimal conditions for synthesis of AgNPs. When aqueous silver nitrate solution is mixed with aqueous propolis extract, the silver ions are rapidly reduced, resulting in the production of extremely stable, crystalline silver nanoparticles in solution. The biosynthesized silver nanoparticles were characterized using different techniques (FTIR, XRD, Zeta potential, TEM, SEM, and EDX). The sizes of the as synthesized silver nanoparticles were 9.43 and 11.39 nm, according to TEM and XRD analysis, respectively. A negative Zeta potential of -8.8 mV were obtained suggesting stabilization of the AgNPs colloidal solution. The antimicrobial activity of biosynthesized silver nanoparticles against Staphylococcus aureus and Escherichia coli was examined and showed a synergism between propolis and AgNPs as expected for gram-negative bacteria Escherichia coli while there was no effect for the extract at the concentrations used. The results proved that biosynthesized AgNPs using Egyptian propolis is promising to be an effective antimicrobial product to be used in infections.
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