Green Synthesis of Silver Nanoparticles using Egyptian Propolis Extract and its Antimicrobial Activity

Document Type : Original Article


1 Physics Department, Faculty of Science, Ibb University,Ibb, Yemen

2 Chemistry Department, Faculty of Science, Ain Shams University, Abbassia, 11566, Cairo, Egypt

3 Chemistry Department, Faculty of Science, Ain Shams University


There is great research interest in synthesizing nanomaterials using environmentally friendly methods due to the many ‎applications of these materials in several fields. In this paper, a facile and green chemistry method was used to synthesize silver ‎nanoparticles (AgNPs) using the Egyptian propolis as reducing and capping agent. Different parameters such as aqueous ‎propolis extract quantity, pH, reaction time, and temperature were investigated to get the optimal conditions for synthesis of ‎AgNPs. When aqueous silver nitrate solution is mixed with aqueous propolis extract, the silver ions are rapidly reduced, ‎resulting in the production of extremely stable, crystalline silver nanoparticles in solution. The biosynthesized silver nanoparticles ‎were characterized using different techniques (FTIR, XRD, Zeta potential, TEM, SEM, and EDX). The sizes of the as ‎synthesized silver nanoparticles were 9.43 and 11.39 nm, according to TEM and XRD analysis, respectively. A negative Zeta ‎potential of -8.8 mV were obtained suggesting stabilization of the AgNPs colloidal solution. The antimicrobial activity of ‎biosynthesized silver nanoparticles against Staphylococcus aureus and Escherichia coli was examined and showed a synergism ‎between propolis and AgNPs as expected for gram-negative bacteria Escherichia coli while there was no effect for the extract at ‎the concentrations used. The results proved that biosynthesized AgNPs using Egyptian propolis is promising to be an effective ‎antimicrobial product to be used in infections.‎


Main Subjects

  • Receive Date: 06 November 2021
  • Revise Date: 07 December 2021
  • Accept Date: 12 December 2021
  • First Publish Date: 12 December 2021