Influence of Light Quality on Growth and Secondary Metabolite in Tissue Cultures of Gardenia jasmonides, Variegata

Document Type : Original Article

Authors

1 Department of Plant Biotechnology, Biotechnology Research Institute, National Research Centre (NRC), Cairo 12622, Egypt

2 Department of Genetics and Cytology, Biotechnology Research Institute, National Research Centre (NRC), Cairo 12622, Egypt

Abstract

The influence of white, blue and red Light-Emitting Diodes (LED) on the growth and the secondary metabolites production in calli and shoots cultures of Gardenia jasmonides, Variegata was evaluated. The white light generally has a powerful effect on both fresh and dry weights of the two types of cultures. White light treatment recorded (11.88 and 0.63 g) for the fresh and dry weight (in case of calli) followed by the blue light which recorded (11.78 and 0.60 g ), respectively. The extracts of shoots exposed to white LED light recorded the highest reducing efficiency (74.40 %). It could be justified as the shoots that exposed to the white light recorded the highest cholorogenic acid (CGA) compared to all the other treatments. Among different phenolic and flavonoids compounds which were tested in the calli and shoots cultures exposed to the different LED light quality, only the chlorogenic acid was detected in the different treatments in both cultures with varying quantities. The white LED light recorded the highest content of chlorogenic acid with both cultures, since it scored 52.43 µg / gdw with calli cultures versus 8783.53 µg / gdw with shoots cultures.

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History

  • Receive Date: 20 October 2021
  • Revise Date: 01 November 2021
  • Accept Date: 02 November 2021

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