Fermentation of Multi-Targeted Products: A statistical Approach Discussed the Production of Cell Wall Hydrolytic Enzymes from Streptomyces rochei for Pathogenic Fungi biocontrol

Document Type : Original Article

Authors

Chemistry of Natural and Microbial Products Department, National Research Centre, (ID: 60014618), 33-El Bohouth st., Dokki, Giza, Egypt, P.O.12622

Abstract

The production of synergistically multi-acting cell wall hydrolyzing enzymes such as chitinase, glucanase, protease and whole cell-wall lytic enzymes “WCL” was highly observed in the culture filtrate of a strain molecularly characterized as Streptomyces rochei MZ227230. Enzymes productivity was monitored in different media and superiority of the modified lytic enzymes production medium (M4) was clearly noticed. Then the simultaneous optimization production of these enzymes through application of statistical modelling was conducted. Box-Behnken design (BBD) using the key components of M4 (C. albicans cell walls, baker yeast, chitin and peptone) followed by independent analysis for productivity of each enzyme helped to conduct four significant models describing four media that were specifically optimized to produce the utmost yields of chitinase (14.97), glucanase (27.89), protease (137.59) and WCL (65.28 U/ml). Pearson’s correlation analysis indicated the impossibility to potentiate the tested enzymes to the utmost yields simultaneously. However, the coproduction medium potentiated more than 80% of the maximal yields for all enzymes. Furthermore, the crude filtrate showed potent fungicidal effect against Fusarium graminearum, Mucor racemosus, Fusarium solani, and Candida albicans which should be attributed to its hydrolytic activity as indicated by the dose and time-dependent release of DNA, RNA and protein constituents of C. albicans

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History

  • Receive Date: 10 July 2021
  • Revise Date: 26 July 2021
  • Accept Date: 28 July 2021

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