Document Type : Original Article
Authors
1
National research centre, Nutrition and food Science Department
2
Nutrition and Food Science Department, National Research Centre, 12622, Giza, Egypt.
3
Pharmacognosy Department, National Research Centre, 12622, Giza, Egypt.
4
Hormones Department, Medical Research Division, 2Stem Cell Laboratory, Center of Excellence for Advanced Science, National Research Centre, 12622, Giza, Egypt.
5
Nutrition and Food Science Department, National Research Centre, 12622, Giza, Egypt
6
Department of Biochemistry, Faculty of Agriculture, Cairo University, 12613, Giza, Egypt.
Abstract
The objective of this study was to evaluate the potential toxicity of methanolic extracts of Pinus roxburghii branch (P. roxburghii) and Nauplius graveolens (N. graveolens). Single oral dosage was administered to male mice at increasing concentrations of 100, 250,500,1000 and 2000 mg/kg body weight to assess the median LD50, which causes the death of 50% (one half) of the mice. Blood samples were collected at the end of the 14 day toxicological trial for the liver and kidney function tests. The results showed that the LD50 of P. roxburghii was 1708.3 mg/ kg body weight and of N. graveolens 1562.5 mg/kg body weight. The acute toxicity had detrimental effects on the liver and kidney function. Based on this finding, a second trial was designed, and the mice were administered day after day intragastrically one tenth (1/10) the LD50 of each plant extract for a whole month. At the termination of the 30-day trial, blood samples and organs were collected for liver and kidney function tests. The long-term administration of 1/10 the LD50 dosages had no significant adverse effects neither on the liver and kidney functions nor on the histopathological examination. In conclusion, further studies are warranted to determine the potential application of these plant as natural therapeutic products.
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