Zekri, N., Zerkani, H., Elazzouzi, H., Zair, T., El Belghiti, M. (2021). Extracts of M. pulegium (L.) and M. spicata(L.): Effect of Extraction Conditions on Phenolics and Flavonoïds Contents and Their Antioxidant Power. Egyptian Journal of Chemistry, 64(3), 1447-1459. doi: 10.21608/ejchem.2020.43922.2893
Nadia Zekri; Hannou Zerkani; Hanane Elazzouzi; Touria Zair; Mohammed El Belghiti. "Extracts of M. pulegium (L.) and M. spicata(L.): Effect of Extraction Conditions on Phenolics and Flavonoïds Contents and Their Antioxidant Power". Egyptian Journal of Chemistry, 64, 3, 2021, 1447-1459. doi: 10.21608/ejchem.2020.43922.2893
Zekri, N., Zerkani, H., Elazzouzi, H., Zair, T., El Belghiti, M. (2021). 'Extracts of M. pulegium (L.) and M. spicata(L.): Effect of Extraction Conditions on Phenolics and Flavonoïds Contents and Their Antioxidant Power', Egyptian Journal of Chemistry, 64(3), pp. 1447-1459. doi: 10.21608/ejchem.2020.43922.2893
Zekri, N., Zerkani, H., Elazzouzi, H., Zair, T., El Belghiti, M. Extracts of M. pulegium (L.) and M. spicata(L.): Effect of Extraction Conditions on Phenolics and Flavonoïds Contents and Their Antioxidant Power. Egyptian Journal of Chemistry, 2021; 64(3): 1447-1459. doi: 10.21608/ejchem.2020.43922.2893
Extracts of M. pulegium (L.) and M. spicata(L.): Effect of Extraction Conditions on Phenolics and Flavonoïds Contents and Their Antioxidant Power
2Department of chemisty University of sciences Moulay ismail Meknes Morocco
3Department of chemistry university of sciences Med V Rabat Morocco
4Department of chemistry university moulay ismail of sciences Meknes Morocco
5Department of chemistry university of sciences University Med V Rabat Morocco
Receive Date: 24 September 2020,
Revise Date: 20 November 2020,
Accept Date: 09 December 2020
Abstract
The correlation between the contents of polyphenols and flavonoïds and the degree of antioxidant activity of various extracts from M. pulegium (L.) and M. spicata (L.), obtained by different solvents and methods, was investigated. The crude extracts were prepared by mixing areal parts in powder with methanol/water solution. They were subjected later to liquid-liquid extraction via solvents with progressive polarity (chloroform, ethyl acetate and n-butanol) by maceration and soxhlet techniques. The total phenol and flavonoïds contents from crude extracts and their fractions were determined by using Folin-Ciocalteu and AlCl3 assays respectively. The antioxidant activity of extracts was evaluated by DPPH• (1,1-Diphenyl-2-picrylhydrazyl) radical scavenging test. This activity was measured by inhibition concentration 50% (IC50) values. Generally, higher extract yields were obtained by the soxhlet extraction technique; the crude extracts recorded the best yields for M. pulegium by soxhlet (26.37%) and maceration (13%) while for M. spicata, the aqueous extract by soxhlet (34.9%) and crude one by maceration (9.4%) showed the higher yields. The higher phenolic and flavonoïds contents were found in crude extracts by maceration for both mints whereas by soxhlet, the ethyl acetate and/or n-butanol extracts demonstrated the strongest contents. These extracts, rich in flavonoïds, showed a positive correlation since they have exhibited better antioxidant activity compared to ascorbic acid as the antioxidant reference (IC50= 0.051 mg/ml).
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