Inhibition of Anabaena sp. and Oscillatoria sp. Using Probiotic Lactic Acid Bacteria

Abd ElTawab, Marwa I.; Abdel Gawad, Fagr; Shaban, Ahmed M.; Bassem, Samah; Guerriero, Giulia; Goda, Hanan A.; Higazy, Aziz M.

doi:10.21608/ejchem.2020.41997.2848

Inhibition of Anabaena sp. and Oscillatoria sp. Using Probiotic Lactic Acid Bacteria

Document Type : Original Article

Authors

¹ Center of Excellence for Research and Applied Studies for Climate and Sustainable Development (C3SD-NRC), Water Pollution Research Department, CEAS, National Research Center, Egypt

² Water Pollution Dept., Center of Excellence for Advanced Scineces (CEAS), National Research Centre, Giza, Egypt

³ Water Pollution Dep., Centre of Excellence for Advanced Sciences (CEAS), National Research Centre, Giza, Egypt

⁴ Biology Department of, University of Napoli Federico II, Italy.

⁵ Agricultural Microbiology Department., Faculty of Agriculture, Cairo University, Giza, Egypt

10.21608/ejchem.2020.41997.2848

Abstract

Water blooms are a serious environmental problem affecting fresh and marine ecosystem. Application of probiotic bacteria has been introduced as an emerging and eco-friendly tool to regulate or inhibit cyanobacterial blooms. In the present study, we have isolated and morphologically identified cyanobacteria from El Manzala Lake. Afterward, we examined the use of probiotic lactic acid bacteria MT2 (Lactobacillus sakei) isolated from Nile Tilapia (Oreochromis niloticus) intestine in controlling Anabaena sp. and Oscillatoria sp., isolated from El Manzala Lake. Measuring chlorophyll, phycocyanin and biomass concentration were conducted in a co-culture experiment between the selected cyanobacterial isolates and probiotic lactic acid bacteria (MT2). Chlorophyll content, phycocyanin content and biomass concentration of Anabaena sp., and Oscillatoria sp., were decreased significantly in the co-culture experiment with MT2 bacteria. Also, the microscopic examinations showed that MT2 bacterium was able to lysis and degrade the selected cyanobacterial cells under microscope. We concluded that the use of MT2 (Lactobacillus sakei) is potentially effective against some cyanobacteria especially Anabaena sp. and Oscillatoria sp.

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