Document Type : Original Article
Department of Chemistry, College of Science-University of AL-Mustansiriyah- Baghdad-Iraq
chemistry department, collage of science, al mustansiriyah university
chemistry department, collage of science, al mustansiriyah unversity
This study describes the new simple and accurate spectrophotometric method for the determination of paracetamol after the formation of azo dye with a new chromogenic reagent 4 [(2-amino-1,3thiazol-4-yl)amino] nitrobenzene to form an orange-coloured product measured at 425 nm. The molecular structure of the newly synthesized compound was confirmed by several spectroscopic techniques such as UV-visible, FTIR, 1HNMR, and mass spectroscopy. Newly synthesized compound was in vitro screened against several bacterial species. The experimental conditions that affect the reaction were carefully optimized and under the optimized conditions, a linear relationship was obtained in the concentration range of 5–25mg.L−1 of paracetamol. A reaction with a new chromogenic reagent has been occurred at a stoichiometric ratio of 1:1. This method has a limit of detection of 1.334 mg.L-1 and Sandell's sensitivity of 0.0235 mg.cm-1 for new azo dye product.