Spectrophotometric Determination of Paracetamol using a Newly Synthesized Chromogenic Reagent 4-[(2-amino-1, 3thiazol-4-yl)amino]nitro benzene

Document Type : Original Article

Authors

1 Department of Chemistry, College of Science-University of AL-Mustansiriyah- Baghdad-Iraq

2 chemistry department, collage of science, al mustansiriyah university

3 chemistry department, collage of science, al mustansiriyah unversity

Abstract

This study describes the new simple and accurate spectrophotometric method for the determination of paracetamol after the formation of azo dye with a new chromogenic reagent 4 [(2-amino-1,3thiazol-4-yl)amino] nitrobenzene to form an orange-coloured product measured at 425 nm. The molecular structure of the newly synthesized compound was confirmed by several spectroscopic techniques such as UV-visible, FTIR, 1HNMR, and mass spectroscopy. Newly synthesized compound was in vitro screened against several bacterial species. The experimental conditions that affect the reaction were carefully optimized and under the optimized conditions, a linear relationship was obtained in the concentration range of 5–25mg.L−1 of paracetamol. A reaction with a new chromogenic reagent has been occurred at a stoichiometric ratio of 1:1. This method has a limit of detection of 1.334 mg.L-1 and Sandell's sensitivity of 0.0235 mg.cm-1 for new azo dye product.

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Volume 63, Issue 12 - Serial Number 12
December 2020
Pages 4681-4693
  • Receive Date: 20 September 2019
  • Revise Date: 11 October 2019
  • Accept Date: 02 June 2020
  • First Publish Date: 06 June 2020