Saraca indica L. leaves, Phytochemical, invitro Antioxidant and Cytotoxic Activities

Document Type : Original Article

Authors

1 Department of Pharmacognosy, National Research Centre

2 Pharmacognosy Dept. , National Research Centre; 33-Elbohouth St, (Former El-Tahrir St.), Dokki – Giza-Egypt. P.O.12622, ID: 60014618

3 Pharmacognosy Department, Pharmaceutical and Drug Industry, National Research Center

4 pharmacognosy Dept. Faculty of pharmacy Cairo university

5 Pharmacognosy Department, Faculty of Pharmacy, Cairo University

Abstract

Saraca indica L. (Saraca asoca) is reported for its important biological benefits in Indian folk medicine. The powdered leaves were extracted with 70% ethanol to obtain total ethanol extract(TEE) that was successively fractionated to get petroleum ether(pet. ether),chloroform(CHCl3), ethyl acetate (EtOAc) and methanol extracts.The antioxidant activityof TEE by three methods of assay, as well as,the antioxidant activity of TEE and successive extracts by DPPHmethod at two dose were done.Total phenolics and total flavonoids content of the plant were determined. The cytotoxic evaluation revealed that EtOAc extract exhibited activity against HCT-116 andMCF-7, Hela& HEPG-2 . GC/MS analysis of the unasponifiable(USF) and saponifiable (SF)fractions of the non-polar extractwas carried out. 34 Compounds with phytol, dodecane, BHT and α -amyrin as the major compoundsin the USFand 25 compounds with methyl palmitate&Methyl-9, 12,15- octadecatrienoate as major compounds in the SF were identified. Fifteen phenolic compounds were detected in HPLC analysis of EtOAcextract as well as six phenolic compounds were isolated and identified for the first time from the same extract they were; gallic acid, methylgallate, rutin, quercetin -3-O-rhamnoside, quercetin and kaempeferol

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History

  • Receive Date: 28 January 2020
  • Revise Date: 20 February 2020
  • Accept Date: 04 March 2020

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