Modulation of Nephrotoxicity Induced by Gentamicin with Bone Marrow Mesenchymal Stem Cells and Moringa Oleifera Extract

Document Type : Original Article

Authors

1 Department of Pharmacology & Toxicology, Faculty of Pharmacy, Cairo University, Cairo

2 Department of Zoology, Faculty of Science, Arish University, Arish, Egypt

3 Department of Biochemistry, Faculty of Medicine, Cairo University, Cairo, Egypt

4 Department of Food Toxicology & Contaminants. National Research Center

Abstract

The study was undertaken to evaluate the protective effect of bone marrow mesenchymal stem cells (BM-MSCs) and Moringa oleifera extract (MOE) against gentamicin (GN)-induced nephrotoxicity in male albino rats. Thirty two adult male rats were divided into four groups including the control group, the group injected i.p with a single dose of GN (100 mg/kg b.w), the group treated orally with MOE (400 mg/kg b.w) for 6 days then injected with a single dose of GN and the group was injected with a single dose of BM-MSCs (5x105cells) by tail vein then injected with GN. At the end of experiment blood and kidney tissue samples were collected for estimation of different biochemical parameters. The results recorded a significant increase in BUN, serum KIM-1, cystatin C, creatinine, sodium and renal MDA accompanied with a significant decrease in serum calcium, renal GSH, SOD and CAT in GN alone-treated group as compared to control group. Co administration of MOE or BM-MSCs before GN injection improved all above parameters when compared with GN administered group. It could be concluded that MOE and BM-MSCs have a therapeutic and protective action against AKI induced by GN administration which manifested by lowering kidney markers and MDA contents and elevation in antioxidant profile.

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History

  • Receive Date: 17 October 2019
  • Revise Date: 13 November 2019
  • Accept Date: 20 November 2019

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