Oxidative Stress of Some Triazolopyrimidine Derivatives and their Nucleoside Analogues on Mcf-7 and A549 Cell Lines

Document Type : Original Article


1 Biochemistry department- NRC- Egypt

2 Biochemistry department -National Research Center (NRC).

3 biochemistry department, National research center, Egypt.

4 Biology Department, College of Science, Jouf University, Sakaka, 2014, Saudi Arabia.

5 Applied Organic Chemistry Department, National Research Centre, Dokki, Cairo, Egypt.

6 Photochemistry Department, Naional Research Centre, Dokki, Cairo, Egypt


The synthesis and assessment of the biological benefits and applications of new triazolopyrimidines and their glycoside derivatives were discussed and researched thoroughly. Interestingly, the data revealed activity of these newly synthesized compounds in A549 and MCF-7 cell lines, where MCF-7 cells responded more effectively to the examined compounds than the other cell lines. The present work’s aim is to appraise the behavior of some free radical enzymes, including SOD, CAT, GSH-Px and oxidative stress parameters H2O2, NO, GSH in MCF-7 and A549 treated cells. The results brought out that, there is an increase in the level of H2O2 and NO associated with an acme in the SOD enzyme activity, besides there is a diminution in the activities of CAT, GSH and GSH-Px when we compared it with control. These results may indicate that compounds 1, 2, 3 and 4 may exert their anticancer activities - to some extent – through the inflection of both the production of reactive oxygen species (ROS) and the action of antioxidant enzymes


Main Subjects