The synthesis of bimetallic nanoparticles (BNPs) has garnered significant interest due to their superior physicochemical and biological properties compared to monometallic nanoparticles (MNPs). In this study, Ag/Au alloy nanoparticles were synthesized via pulsed laser ablation in liquid (PLAL) and characterized for their optical, morphological, and antibacterial properties. The formation of Ag/Au alloy nanoparticles was characterized through UV-Vis spectroscopy, transmission electron microscopy (TEM), and inductively coupled plasma (ICP) analysis. The antibacterial efficacy of the synthesized nanoparticles was evaluated against Methicillin-Resistant Staphylococcus aureus (MRSA) in vitro. Additionally, a femtosecond laser at 400 nm wavelength was employed to assess the synergistic effects of laser irradiation and Ag/Au alloy nanoparticles on bacterial viability. Growth kinetics analysis revealed that femtosecond laser treatment alone significantly reduced bacterial proliferation (P < 0.0001), whereas Ag/Au alloy nanoparticles required higher concentrations to exhibit noticeable antibacterial effects. The combination of both approaches resulted in the most pronounced bacterial growth inhibition. This study unlocked a new perception of synthesizing Ag/Au alloy nanoparticles via the laser ablation technique and their potential application in treating various ocular infections particularly when used in conjunction with femtosecond laser-based antimicrobial photodynamic therapy. To our knowledge, this is the first study to report the combined use of femtosecond laser irradiation and PLAL-synthesized Ag/Au alloy NPs for antimicrobial photodynamic therapy, offering a novel approach to combating drug-resistant eye pathogens. Further studies are needed to optimize the synthesis parameters and evaluate the biocompatibility of these nanoparticles for future clinical applications.
El-Gendy, A., Hussein, T., Ahmed, E., Dabbous, O., & Mohamed, T. (2025). Antibacterial Potential of Ag–Au Alloy Nanoparticles Combined with Femtosecond Laser Light Against Drug-Resistant Eye Pathogens. Egyptian Journal of Chemistry, 68(13), 911-921. doi: 10.21608/ejchem.2025.374399.11575
MLA
Ahmed El-Gendy; Talal A Hussein; Esraa Ahmed; Ola Dabbous; Tarek A Mohamed. "Antibacterial Potential of Ag–Au Alloy Nanoparticles Combined with Femtosecond Laser Light Against Drug-Resistant Eye Pathogens", Egyptian Journal of Chemistry, 68, 13, 2025, 911-921. doi: 10.21608/ejchem.2025.374399.11575
HARVARD
El-Gendy, A., Hussein, T., Ahmed, E., Dabbous, O., Mohamed, T. (2025). 'Antibacterial Potential of Ag–Au Alloy Nanoparticles Combined with Femtosecond Laser Light Against Drug-Resistant Eye Pathogens', Egyptian Journal of Chemistry, 68(13), pp. 911-921. doi: 10.21608/ejchem.2025.374399.11575
VANCOUVER
El-Gendy, A., Hussein, T., Ahmed, E., Dabbous, O., Mohamed, T. Antibacterial Potential of Ag–Au Alloy Nanoparticles Combined with Femtosecond Laser Light Against Drug-Resistant Eye Pathogens. Egyptian Journal of Chemistry, 2025; 68(13): 911-921. doi: 10.21608/ejchem.2025.374399.11575
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