Partial Purification, Stability and Application of α Amylase Produced by Ochrobacterium anthropi

Document Type : Original Article

Authors

Chemistry of Natural and Microbial Products Department, Pharmaceutical and Drug Industries Research Institute, National Research Centre, ElBuhous St. 12622, Dokki, Giza, Egypt.

Abstract

Amylases that hydrolyze starch are essential enzymes utilized in the food, textile, detergent, and pharmaceutical sectors. Although numerous fungi, yeasts, bacteria, and actinomycetes have produced alpha amylase, the majority of industrial uses have been for enzymes obtained from bacterial and fungal sources. In the present study α amylase was produced by the newly local isolate Ochrobacterium anthropi . Culture condition optimization showed that high amylase activity of the enzyme was characterized by expressing the optimum activity at 60oC and pH 7.0. It showed thermal stability after 1h where relative enzyme activity was 97.1%. The pH stability covered a range starting from 6.0-9.0 for 1h. The purification studies were carried out using ammonium sulphate. The best activity was obtained by partially purified using 80% ammonium sulphate precipitation technique and the purification fold was 1.49%. The Km value for the enzymatic hydrolysis of starch was 5.5mM.The partially purified amylase was immobilized in sodium alginate (2%w\v) for application in detergent formulation and was proven to be much better at removing stains from cotton textiles than the other detergent that was tested. amylase was quite effective in removing chocolate stains when used with different commercial detergent (Persil, Fairy & Pril). When compared to detergent alone, the wash performance examination of chocolate stains on cotton fabric revealed a higher reflectance with detergent plus enzyme.

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Egyptian Journal of Chemistry
Volume 68, Issue 13 - Serial Number 13
(In Loving Memory of Late Professor Doctor”Zeinab M. Nofal” In progress
December 2025
Pages 935-944

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History

  • Receive Date: 12 April 2025
  • Revise Date: 14 May 2025
  • Accept Date: 10 June 2025

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