Document Type : Original Article
Authors
1
Biochemistry division, Chemistry department, Faculty of science, El fayoum university
2
Physics Dept., Medical Biophysics Division Faculty of Science Helwan University
3
Medical Retina Department, Research Institute of Ophthalmology, Giza, Egypt.
4
Chemistry Department, Biochemistry division, Faculty of Science, El Fayoum University, El Fayoum, Egypt.
5
Biochemistry and Pharmaceutical Unit -Research Institute of Ophthalmology, Giza, Egypt
Abstract
Diabetic cataracts are linked to oxidative stress, so to slow cataract progression, antioxidants should be used as therapy. Chrysin is a natural polyphenol has anti-inflammatory, antioxidant and antidiabetic effect with low bioavailability. Liposomes are nano-carriers for drug delivery. Our goal is to prepare Chrysin loaded liposome (CLL) ocular drug and study its potential as a topical medication, also compare with bioactivity of oral chrysin administration to treat diabetic cataracts. The thin lipid film method was used to synthesize CLL. Eighty male rats were divided into control, STZ- induced diabetic, Local and systemic treated groups. The systemic group received chyrsin orally once daily solubilized in 0.1% DMSO, whereas the treated group received CLL eye drop twice daily for 12 weeks. At end of experiment, slit-lamp imaging and lens examination were done. GSH, TSP, CP, Calpain, Caspase-3, and Ca were assessed in the lens tissue. There was significant difference of all parameters in treated groups compared to STZ diabetic group. An efficient CLL preparation was demonstrated by the results of DSC, TEM imaging, particle size, and zeta potential. In the lens tissue of diabetic rats, CLL demonstrated antioxidant effectiveness in oxidative stress-related parameters than systemic orally treatment. Lastly, the results showed that formulated chrysin might be suggested as a potential ocular drug for diabetes cataracts.
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