Document Type : Original Article
Authors
1
Therapeutic Chemistry Department, Pharmaceutical Industries and Drug Research Institute, National Research Centre; Dokki 12622, Cairo, Egypt
2
Therapeutic Chemistry Department, Pharmaceutical Industries and Drug Research Institute, National Research Centre; Dokki 12622, Cairo, EgyptNational Research Center
3
Cell Biology Department, Biotechnology Research Institute, National Research Centre; Dokki 12622, Cairo, Egypt
4
Chemistry of Natural Compounds Department, Pharmaceutical and Drug Industries Research Institute, National Research Centre, Giza, Egypt
Abstract
The most frequent and dangerous consequence of diabetes mellitus is diabetic nephropathy (DN), which is the primary cause of chronic kidney damage. Oxidative stress is a key player in the pathophysiology of diabetic nephropathy. The purpose of this study was to employ naturally occurring products that have a strong ability to prevent oxidative stress, such as purslane (Portulaca oleracea) extracts. By chromatographic techniques, seven compounds were isolated from the purslane seeds following a phytochemical examination. The constituents were identified through spectroscopic data analysis. Seventy rats were divided into seven groups including control, purslane seeds methanol and methylene chloride extracts, DN), DN plus two extracts and DN plus losartan. Purslane methanol and methylene chloride extracts against DN were evaluated to the DNA fragmentation, expression levels of diabetes and nephrotoxicity related genes, the activity of GPx, SOD, GST enzymes, NO level, the apoptosis, and ROS generation. DN rats showed increased in DNA fragmentation, rise in the expression levels of diabetes and nephrotoxicity related genes, decline in the tested antioxidant enzymes activity and elevation of the NO level and apoptosis. Treatment of DN rats with two extracts of purslane improved the harmful effects that appeared in the DN rats, but the efficiency of the methanolic extract was much higher than those in methylene chloride extract. These results suggested that the reduction of oxidative stress after treatment with P. oleracea extracts can be due to the presence of antioxidant compounds such as flavonoids and polyphenols which scavenge the free radical.
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