Biogenesis of Silver Nanoparticles Feasible for Industrial Application Using Yeast-fungi Secreted Enzymes and Proteins

Document Type : Original Article


1 Chemistry of Natural and Microbial products department, National Research Center, Dokki, Cairo, Egypt

2 Department of Chemistry, Faculty of Science, Helwan University, Ain-Helwan, Cairo, Egypt

3 Textile Research Division, Dokki, Cairo, Egypt. National Research Center,


Saccharomyces cerevisiae, Rhodotorula glutinis and Geotrichum candidum were selected for biosgenesis of silver nanoparticles (AgNPs) using yeast reductases. Extracellular proteins act as stabilizers for so formed AgNPs. Factors affecting biosynthesis ; biomass concentration, AgNO3 concentration and the ratio of AgNO3: to biomass concentration on. The Optimum conditions for biosynthesis of AgNPs could be attained using biomass of Saccharomyces cerevisiae (3 mL/100 mL): .9 mM AgNO3: pH 12: 25 ◦C and 24 h incubation). Under these conditions, the maximum concentration of well stabilized AgNPs obtained was 900 ppm with a mean diameter range of 1.5–12 nm. Such solution is unequivocally feasible for industrial applications.


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