Document Type : Original Article
Authors
1
Department of Biotechnology and Life Sciences, Faculty of Postgraduate Studies for Advanced Sciences, Beni-Suef University, Beni-Suef, 62511, Egypt.
2
Forensic Medicine and Toxicology Department, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef, 62511, Egypt
3
Department of Biochemistry and Molecular biology, Faculty of Veterinary Medicine,Cairo University, Cairo, Egypt
4
Doctor of Inorganic Chemistry at Al-Nasr Company for Intermediate Chemicals, National Service Agency-Ministry of Defense
5
Biochemistry Department, Faculty of Agriculture, Al –Azhar University, Cairo, Egypt.
Abstract
Background
This study examined whether local application of natural products (ginseng extract, ginseng-loaded ZnONPs, and ZnONPs alone) can effectively prevent dermatitis in healthcare workers exposed to glutaraldehyde 2% disinfectant during infection control procedures.
Materials and methods
The experimental design comprised five female mice groups. Group 1 (G1) served as the control group without treatment. Group 2 (G2) was exposed to 2% glutaraldehyde (GLU) spray at a dose of 2 mg/kg body weight. Group 3 (G3) received GLU spray plus topical ginseng extract cream. Group 4 (G4) was treated with GLU spray plus topical zinc oxide nanoparticle (ZnONP) cream. Group 5 (G5) administered GLU spray plus topical ginseng-loaded ZnONP cream. The treatment regimen was administered five days per week over a 30-day period for all experimental animals.
Results
On the basis of our results, glutaraldehyde-induced dermatitis changed the histological appearance as well as the biochemical parameters by increasing the mRNA expression of TNF-α, IL-1b, nf-kb 1, and ptgs2, and downregulating the mRNA expression of TGFB1.
Conclusion
Treatment with ginseng-ZnONPs and ZnONPs had a preventive effect on glutaraldehyde across the suppression of oxidative stress and inflammation markers and the augmentation of antioxidants.
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