Impact of random amplified polymorphic DNA analysis as a genetic crossmatching tool for human kidney transplantation.

Mahmoud, Ashraf Zakaria; EL-Sawi, Mamdouh; Fayed, Shimaa M.

doi:10.21608/ejchem.2025.363825.11369

Impact of random amplified polymorphic DNA analysis as a genetic crossmatching tool for human kidney transplantation.

Articles in Press

Document Type : Original Article

Authors

¹ Urology and Nephrology center, Faculty of medicine, Mansoura university

² Department of Zoology, Faculty of Science, Mansoura University, Mansoura, Egypt.

³ Urology and Nephrology center, Faculty of science, Mansoura university

10.21608/ejchem.2025.363825.11369

Abstract

Renal failure was a condition that one or both of kidneys not work their job. Kidney transplantation and hemodialysis are the main rescue of the kidney failure patients. The limitation of conventional transplantation crossmatching tests is the false positive results that are up to 50%. So; the study was attempted to produce a complementary sharp sensitive molecular method for kidney transplantation crossmatching; it is random amplified polymorphic DNA (RAPD) analysis. The study included 21chronic kidney failure patients, with their donors. Histopathological results showed that; most of the patients were with chronic pyelonephritis kidneys. Biochemical results appeared that sodium, aspartate aminotransferase and albumin values were decreased significantly post kidney transplantation, but cholesterol values were increased significantly. Complete blood count illustrated significant decreasing of each of red blood cells, hemoglobin, mean corpuscular volume, hematocrit %, and platelets levels pre and post transplantation. Urine analysis results expressed significant increasing in protein, red blood cells and puss cells values pre transplantation. RAPD analysis was produced two marker primers A-04 and A-05 succeeded in producing identical banding pattern of each patient and his donor with total a similarity (P=1) and going with the conventional crossmatching tests results like, blood grouping and human leukocyte antigen.

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