Phytochemical Profiling and Assessment of Antibacterial Activity of Linaria Tarhunensis Pamp A Novel Investigation

Atayalla, Khamis A.; Ackacha, Mohamed A.; Zahran, Doaa R.

doi:10.21608/ejchem.2025.363181.11354

Phytochemical Profiling and Assessment of Antibacterial Activity of Linaria Tarhunensis Pamp A Novel Investigation

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Document Type : Original Article

Authors

¹ Department of Chemistry, Faculty of Science, Sebha University, Sebha, Libya

² Department of Chemistry, Faculty of Science, Bani Waleed University, Libya

³ Department of Microbiology, Faculty of Science, Cairo University, Giza, Egypt

10.21608/ejchem.2025.363181.11354

Abstract

Linaria species have been used for a wide range of therapeutic uses. Linaria Tarhunensis Pamp belongs to the genus Linaria, within the family Plantaginaceae, a plant native to Libya that has not been investigated before.The objectives of the current work are to identify the phytochemical, biological effects of Linaria Tarhunensis Pamp extracts using a slightly modified maceration method via two different solvents and to determine its total phenolic contents (TPC) and total flavonoid contents (TFC) employing spectrophotometric methods. Total phenolic compounds were measured using Folin-Ciocalteu reagent and total flavonoids were measured using a quercetin reference standard method. Qualitative screen for the presence of major classes of phytochemical constituent's, alkaloids, flavonoids, phenolics, tannins, saponins, steroids, terpenoids, carbohydrates and glycosides were done according to standard processes. All of the tested compounds were present in the phytochemical analysis except for saponins and tannins. The results indicated that the methanol extract had a high quantity of polyphenols (29.63 mg QE/g) and flavonoids (21.41 mg QE/g), while the chloroform extract contained (11.63 mg QE/g of TFC and 16.56 mg QE/g of TPC). The antibacterial activity of the studied extracts proved ineffective against the used bacterium strains MRSA, S. epidermidis, and S. aureus even though, they might be used as antioxidant or anti-inflammatory

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