Document Type : Original Article
Authors
1
Professor of Cell biology and Genetics. Department of Zoology, Faculty of Science, Tanta University
2
Biochemistry Division, Chemistry Department, Faculty of Science, Tanta University, Tanta, Egypt.
3
Biochemistry Division, Chemistry Department, Faculty of Science, Tanta University, Tanta 31527, Egypt.
Abstract
The present investigation examined the potential antioxidant and cytotoxic impact of Balanites aegyptiaca extract (BAE) against A549 lung cancer cells in vitro using a Sulforhodamine B (SRB) assay. After extraction and characterization with GC-MS chromatography, which showed the principal components of the BAE, and the evaluation of the total antioxidant capacity of BAE that indicated a clear antioxidant activity, the median inhibitory concentration (IC50) of A549 cancer cells was determined. Four groups of cells were treated: Group 1 (G1); Untreated control, Group 2 (G2); BAE alone, Group 3 (G3); Gemcitabine (Gem) alone, and Group 4 (G4); Combination of Gem + BAE. (1:1 volume). The results showed that the treatment with BAE, Gem, or their combination reduced the numbers of A549 viable cells in a concentration-dependent manner and induced DNA fragmentation, causing clear cellular toxicity and apoptosis. After treatment, the cells showed shrinkage, rounding, detaching, floating, swelling, and rupture, exhibiting apoptotic features. The combination treatment has shown more obvious apoptotic topographies. Furthermore, the lipid peroxidation levels in A549 lung cancer cells were significantly decreased by treatment, the antioxidative stress enzyme activities of super oxide dismutase (SOD), glutathione S transferase (GST), and glutathione peroxidase (GPX), were increased and the catalase (Cat) activity was down-regulated in all treatments. In conclusion, BA extract induced dual antioxidant and apoptotic effects indicated by strong DNA fragmentation; both enhanced Gem's activity when administered in combination.
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