Association of Micro-RNAs (320-b, 101-3p) and Parvovirus B19 with Re- current Miscarriage

Elsayed, Ahmed A.; Shoman, Sahar A.; Farag, Mona K.; Shaker, Mai M.; Gaber, Khaled R.

doi:10.21608/ejchem.2025.338036.10901

Association of Micro-RNAs (320-b, 101-3p) and Parvovirus B19 with Re- current Miscarriage

Document Type : Original Article

Authors

¹ Prenatal diagnosis and Fetal Medicine Department, Human Genetics and Genome Research Institute, National Research Centre Cairo, Egypt.

² Microbiology Department, Faculty of Science, Ain Shams University, Cairo Egypt.

10.21608/ejchem.2025.338036.10901

Abstract

Miscarriage is the spontaneous end of pregnancy before 20 weeks of gestation. miRNAs serve as important regulators of target genes to control different pathophysiological events. Parvovirus B19 is a human pathogenic virus. Non-immune pregnant women are at risk for fetal infection with greater complications if transmission occurs in the first or second trimester. Here we try to study the expression of micro RNAs (320-b and 101-3p) and b) to detect the incidence of Parvovirus B19 antibodies among pregnant women with recurrent miscarriage. A prospective study comprised 80 pregnant women from the Prenatal Diagnosis and Fetal Medicine Department, National Research Centre, Egypt between January 2022 and March 2024 divided into the case group including 40 pregnant women with 2 or more histories of recurrent miscarriages and presenting with threatened abortion at gestational age 6 – 14 weeks. The control group consisted of 40 pregnant women with no history of miscarriages and at least one living child. micro-RNA (320-b and 101-3p) expression was done using Real-time PCR. Quantification of parvovirus B19 antibodies IgG and IgM using Enzyme-linked Immunosorbent Assay. Results revealed that miR 320-b was upregulated with a highly significant difference between the control group and the case group (p<0.05), while miR 101-3p was downregulated with a highly significant difference between the control group and the case group (p<0.05). Anti-B19 IgM antibodies (recent infection) tested negative in all subjects, while anti-B19 IgG antibodies (prior infection) were detected in 28.75 % (23/80). In conclusion, miR 320b and miR 101-3p can serve as biomarkers for early diagnosis of recurrent miscarriages. Acute Parvovirus B19 infection was not directly related to miscarriage incidences.

Keywords