Oxidative Stress Biomarkers and NLRP3 Polymorphism in Type 1 Diabetes

Elneely, Enas A.; Saad, Entsar A.; Elsharkawy, Ashraf A.; Toson, Elshahat A.; Attia, Zeinab R.

doi:10.21608/ejchem.2025.327552.10618

Oxidative Stress Biomarkers and NLRP3 Polymorphism in Type 1 Diabetes

Document Type : Original Article

Authors

¹ Chemistry Department, Faculty of Science, Damietta University, Damietta, Egypt

² Department of Pediatrics, Faculty of Medicine, Mansoura University, Mansoura, Egypt

³ Mansoura University Children’s Hospital, Mansoura University, Mansoura, Egypt

10.21608/ejchem.2025.327552.10618

Abstract

Abstract

T1DM is a long-term autoimmune condition impacted by both hereditary and environmental factors. Hyperglycemia encourages oxidative stress creation and activation of multiprotein inflammatory inflammasomes such as NLRP3. We aimed to assess and explain the relationships between the NLRP3 gene's rs10754558 C/G SNP, oxidative stress, and the severity of T1DM in Egyptian pediatrics. 120 T1DM patients and 121 age- and sex-matched healthy participants were included. Real-time PCR was carried out using TaqManTM SNP on the Artus Rotor-Gene Qiagen apparatus for genotyping. Malondialdehyde (MDA) and total antioxidant capacity (TAC) were estimated. Significant elevation in MDA (p<0.001) and reduction in TAC (p<0.001) were noticed. Glycosylated Hemoglobin (HbA1c) was negatively correlated with TAC among the T1DM group (r=-0.313, p<0.001). Serum MDA, TAC, and creatinine all showed elevated risk for predicting T1DM susceptibility, according to logistic regression analysis. The genetic variant of rs10754558 C/G in NLRP3 did not significantly correlate with T1DM susceptibility or MDA or TAC (p>0.05). Among T1DM patients, we confirm MDA elevation and TAC lowering as independent predictors for T1DM susceptibility. Moreover, NLRP3 rs10754558 C/G polymorphism may not be related to oxidative stress markers and disease susceptibility.

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