Combination Quercetin and Poloxamer in a Solid Dispersion Binary System and the Antioxidant Activity Using DPPH and ABTS

Kadir, Abdul; Pradana, Aditya Trias; Budipramana, Krisyanti -; Ratih, Ratih -; Winantari, Agnes Nuniek

doi:10.21608/ejchem.2024.324927.10589

Combination Quercetin and Poloxamer in a Solid Dispersion Binary System and the Antioxidant Activity Using DPPH and ABTS

Document Type : Original Article

Authors

¹ Faculty of Pharmacy, University of Surabaya, Surabaya, 60293 Indonesia

² Department of Pharmaceutics, Faculty of Pharmacy, University of Surabaya, Surabaya 60293, Indonesia

³ Department of Pharmaceutical Biology, Faculty of Pharmacy, University of Surabaya

⁴ Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Surabaya

10.21608/ejchem.2024.324927.10589

Abstract

Free radicals are unstable and highly reactive chemicals. Free radicals are extremely harmful since they may change the DNA sequence and promote the production of cancer cells, heart disease, Alzheimer's, and inflammatory disorders. Substances such as antioxidants are required to neutralize unstable free radicals such as vitamin C, vitamin E, Q10, and flavonoids like quercetin. The characteristic of quercetin is that it is less soluble in water, which limits the effect of antioxidants. This research aims to investigate the incorporation of poloxamer 188 as a catalyst in solid dispersion and to assess its antioxidant properties through the application of 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) methodologies. There were 3 types of solid dispersion formulae (F1, F2, and F3) in this study that were observed in their organoleptic and characterized using particle size analyzers (PSA), differential scanning calorimetry (DSC), and Fourier transform infrared (FTIR). The results showed that F1 had the highest antioxidant content due to its high presence of poloxamer 188. The IC50 of F1 using DPPH and ABTS was 15.92 ppm and 48.57 ppm, respectively.
Keywords : ABTS; Antioxidants; DPPH; Solid Dispersions; Quercetin

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