Green synthesis of selenium nanoparticles using an aqueous extract of in vitro-derived Plectranthus amboinicus leaves has been biosynthesized. To produce in-vitro-derived plants of P. amboinicus to act as an efficient reductive solution for biosynthesizing SeNPs, a new tissue culture protocol was conducted using Spirulina plantensis extract as a natural growth regulator and secondary metabolites elicitor compatible with the adequate concentration of carbon source. Reaching the highest shoot length at 9.0 ± 0.42 and the maximum number of leaves per shoot at 15.0 ± 0.57, the enhancement of total phenolic contents revealed the highest level at 96.4 mg/g on MS supplemented with 1.0 mg of S. plantensis extract and 20 gm of sucrose. The enhancement of total phenolic compounds resulted in biosynthesized SeNPs with spherical-like shape and an average diameter of 17.9 ± 1.34 which was verified with Edx, SEM, TEM, and FTIR analyses. The biosynthesized SeNPs revealed antibacterial activity against the oral pathogens Escherichia coli and Staphylococcus aureus indicating the potential of biosynthesized selenium nanoparticles to be future alternatives to antibiotics losing their activity over time and to be combined with laser applications to intensify their activity in enchanting material delivery to targeted tissues and organs
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