High-throughput Sequencing for differentially expressed microRNAs in Hepatocellular Carcinoma

Eltaweel, Noha; Ezzat, Wafaa; Heiba, Ahmed; Abdelhalim, Ahmed; Mahmoud, Yosra H.; Temraz, Maii Mahmoud; Amr, Khalda

doi:10.21608/ejchem.2024.325959.10579

High-throughput Sequencing for differentially expressed microRNAs in Hepatocellular Carcinoma

Articles in Press

Document Type : Original Article

Authors

¹ Medical Molecular Genetics dept., NRC, Egypt.

² Internal Medicine dept., NRC, Egypt.

³ Hepatology Department, National Hepatology and Tropical Medicine Research Institute.

⁴ Clinical Pathology Department, National Hepatology and Tropical Medicine Research Institute

10.21608/ejchem.2024.325959.10579

Abstract

Background: HCC's has low survival rate this is due to its late-stage diagnosis. Early diagnosis of cancer liver could be successfully managed with high cure rate exceeding 80%. We aimed to search for the top expressed miRNAs that contribute to the pathogenesis in HCC. Furthermore, we performed Target gene prediction and functional annotation of the miRNAs to identify the functional role of the reported miRNAs.

Results: The top DEMs in HCC vs controls were hsa-let-7i-5p, miR-103-3p, miR-24-3p, miR-27a-3p, miR-423-5p and miR-27b-3p. The functional enrichment of those genes revealed the highly enriched KEGG pathways and GO terms.

Conclusion: this study showed the top deregulated miRNAs in plasma of HCC patients, their target genes that might contribute in HCC pathway so they might be highly involved in the disease pathogenesis. Further functional studies are crucial to assess their functions and actual target genes.

Keywords