Modulating effect of green tea and vitamin C in the management of &quot;Alzheimer&#039;s Disease&quot;: oxidative stress, cell-based anti-inflammatory and genotoxic profiles.

Mahmoud, Khaled; El-hallouty, Salwa Mohamed; Hanafy, Mahmoud Khaled; Ayman, Eman; Youssef, Elham Mohamed

doi:10.21608/ejchem.2024.313637.10223

Modulating effect of green tea and vitamin C in the management of "Alzheimer's Disease": oxidative stress, cell-based anti-inflammatory and genotoxic profiles.

Document Type : Original Article

Authors

¹ nrc

² Drug Bioassay-Cell Culture Laboratory, Pharmacognosy Department, National Research Center (NRC), Dokki, Giza, Egypt,

³ Research Centre for Idling brain science, Department of Biochemistry, Graduate School of Medicine and pharmaceutical science, University of Toyama, 930-0194, Japan.

⁴ Faculty of biotechnology, MSA university, Egypt

⁵ Biochemistry Department, National Research Centre

10.21608/ejchem.2024.313637.10223

Abstract

Background:

Alzheimer's disease is a progressive brain disorder. Green tea (GT) and vitamin C are known for their antioxidant and anti-inflammatory properties. This study aims to investigate the potential oxidative stress, cell-based anti-inflammatory and genotoxic Profiles for synergistic effect of green tea and vitamin c against to Alzheimer's disease.

Methods:

In vitro assessments included their several antioxidant parameters and ability to inhibit inflammatory markers such as nitric oxide (NO), interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α), and matrix metallopeptidase 1(MPP1) in lipopolysaccharide-activated macrophages. In vivo approach evaluated genotoxicity and their potential neurotherapy against Alzheimer's disease using a rat model. Behavioral, biochemical, and histological evaluations were conducted.

Results:

Green tea methanolic extract (GTME) and vitamin C showed enhanced antioxidant and anti-inflammatory modulator activities. The neurotoxicity rat’s model exhibited cognitive decline and brain injury in contrast to the control group. While treated rats with GTME and vitamin C exhibited improved cognitive function, behavior and reduced AChE levels the combination exerted a significantly more potent effect. Histological analysis confirmed improved brain tissue structure in rats receiving the combined treatment. Crucially, neither compound exhibited genotoxic properties.

Conclusion: The combination of GTME and vitamin C demonstrated a promising therapeutic potential for neurodegenerative diseases due to their synergistic effects.

Keywords