Document Type : Original Article
Authors
1
Ornamental plants and woody trees, national research centre, Giza, Egypt.
2
Ornamental Plants Woody Trees Department, Agriculture and Biological Research Institute, National Research Centre (NRC), 33 El Buhouth St and., Dokki, Giza, 12622, Egypt.
3
Ornamental plant and Woody tree Dept. Agricultural and Biological Res. Division, National Research Centre (NRC), Egypt.
4
Agricultural Botany Department, Faculty of Agriculture, Cairo University, El-Gamaa St, Giza, 12613, Egypt.
Abstract
Salinity interferes with plant morphological and biochemical functions, which eventually results in serious losses. In vitro shooting and rooting abilities of Khaya senegalensis Desr A. Juss were examined under four levels of salinity stress (0, 1000, 3000, and 5000 ppm) and treated by sodium nitroprusside (SNP) at 0.0, 1.5, 3.0 and 6.0 mg l-1 to alleviate the adverse effect of salt stress. Using SNP in the culture media under salinity levels attained to 3000 ppm had a stimulation impact on in vitro grown and survived plants, shootlets number, length, and rooting percent. Increasing SNP level to 6 mg l-1 in the salinized culture media at all tested levels (0-5000 ppm) enhanced photosynthetic pigments content as well as the estimated minerals (N, P, K, Ca%, and K+/Na+ ratio), while had an inhibitory effect on Na and Cl%, total phenols and proline under the same salinity level. Leaf and stem transverse sections were showed that salt stress induced an adverse effect on their anatomical traits, however SNP at 6.0 mg l-1 mitigated the harmful effects of salinity stress on histological characteristics of leaf i.e. thickness of leaf midvein & lamina, xylem & phloem tissues, xylem vessels diameter, palisade and spongy tissue thickness, as well as epidermis, cortex, fibers, phloem and xylem tissues thickness for stem structure.
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