Facile one-pot strategy for brain imaging using radiolabeled [99mTc]-tricarbonyl histamine complex in Mice.

Sanad, M.H.; Eyssa, H. M.; Challan, Safaa B.; Farag, A. B.; Abdou, Fatma Y.; Soliman, Aiten M.; Abd-Elhaliem, S. M.; El Bakary, Nermeen M.

doi:10.21608/ejchem.2024.283339.9602

Facile one-pot strategy for brain imaging using radiolabeled [99mTc]-tricarbonyl histamine complex in Mice.

Document Type : Original Article

Authors

¹ Labeled Compound Department, Hot Laboratory Centre, Egyptian Atomic Energy Authority, P.O. Box 13755, Cairo, Egypt

² Radiation Chemistry Department, National Centre for Radiation Research and Technology, Egyptian Atomic Energy, Authority, P.O. Box 11787, Cairo, Egypt.

³ Pharmaceutical Chemistry Department, Faculty of Pharmacy, Ahram Canadian University, Giza, 12578 Egypt.

⁴ Drug Radiation Research Department, National Centre for Radiation Research and Technology, Egyptian Atomic Energy, Authority, P.O. Box 11787, Cairo, Egypt.

⁵ Radiation Biology Department, National Centre for Radiation Research and Technology, Egyptian Atomic Energy Authority, P.O. Box 11787, Cairo, Egypt.

10.21608/ejchem.2024.283339.9602

Abstract

This investigation aims to label the histamine ligand, 2-(1H-imidazol-4-yl) ethanamine, using a [99mTc]-tricarbonyl core method for brain imaging. The optimization of all variables and factors influencing the radiochemical reaction is carried out to produce the labeled compound with a high radiochemical yield of 98.5%. The radiolabeled compound was stable for up to 6 hours, with a logarithm of the partition coefficient value at 1.49 ± 0.04. This value suggests the lipophilic characteristics of the [99mTc]-tricarbonyl histamine compound, indicating its potential to cross the blood-brain barrier effectively. The bioevaluation of the radiotracer [99mTc]-tricarbonyl-histamine complex exhibited a notable brain uptake of 8.5% ID/organ at 5 minutes post-injection and, eliminated through the urinary system within 2 hours post-injection. This study explores the promising developments in brain imaging facilitated by the [99mTc]-tricarbonyl histamine complex.

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