Volatiles composition of fresh aroma and hydrodistilled volatile oil of Chrysopogon zizanioides roots growing in Egypt along with the cytotoxic activities of the hydrodistilled oil

Arafat, Mahmoud; Khalil, Mohammed; Mahrous, Engy A.; El Hawary, Seham

doi:10.21608/ejchem.2024.269434.9311

Volatiles composition of fresh aroma and hydrodistilled volatile oil of Chrysopogon zizanioides roots growing in Egypt along with the cytotoxic activities of the hydrodistilled oil

Document Type : Original Article

Authors

¹ Pharmacognosy department, Pharmacy, Heliopolis University, Cairo, Egypt.

² Faulty of Pharmacy Cairo University

³ Pharmacognosy Department, Faculty of Pharmacy, Cairo University

⁴ Department of Pharmacognosy, Faculty of Pharmacy, Cairo University

10.21608/ejchem.2024.269434.9311

Abstract

Chrysopogon zizanioides oil (CZO) is obtained from the roots of the C. zizanioides plant and it is frequently employed as a major odour contributor in the fragrance and aromatherapy industries. The hydrodistilled CZO was analyzed by GC/MS and compared to fresh sample aroma which was analyzed by headspace GC/MS method. The main constituents of the hydrodistilled oil were Khusimol (16.48%), aristol-1(10)en-9-ol (12.5%) and cyclocopacamphenol (7.69%). While headspace-GC/MS analysis identified bicyclo[5.2.0]nonane, 2-methylene-4,8,8-trimethyl-4-vinyl (14.17%), sativene (11.52%) and valencene (9.71%) as the major contributors to its fresh aroma. CZO was found to be active against two human cancer cell lines of lung (A549) and hepatocellular (HepG2) with IC50 32.2 and 37.6 µg/mL respectively using MTT assay. The results showed great similarity between the Egyptian oil and those available in the international market. Further studies are necessary for the full standardization and biological evaluation of the oil.

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