UPLC-ESI-MS Analysis of Secondary Metabolites from Tamarix Aphylla With Its In-Vitro Anti-Inflammatory and Anti-Microbial Activities

Hamed, Manal Mortady; El-Wakil, Eman Ahmed; Abdel-Aziz, Mohamed S.; El Shahed, Zeinab A.; Abdel-Hady, Heba

doi:10.21608/ejchem.2024.271590.9403

UPLC-ESI-MS Analysis of Secondary Metabolites from Tamarix Aphylla With Its In-Vitro Anti-Inflammatory and Anti-Microbial Activities

Document Type : Original Article

Authors

¹ Medicinal Chemistry, Theodor Bilharz Research Institute, 12411,Giza, Egypt

² Microbial Chemistry Departments, Biotechnology Research Institute, National Research Centre, Dokki, Giza, Egypt

³ Chemistry of Natural and Microbial Products pharmaceutical Industries and Drug research Institute, National research centre.

10.21608/ejchem.2024.271590.9403

Abstract

Tamarix aphylla is considered one of the known species of the genus Tamarix. It has been shown to has a various pharmacological property. Our results showed that; the EtOAc and BuOH extracts have the highest phenolic content between the examined extracts. EtOAc extract antimicrobial activity ranged from 27 mm against Escherichia coli, to 19 mm against Aspergillus niger. Moreover, BuOH, EtOAc, and Aqueous extracts proved anti-inflammatory efficacy as induced marked inhibition of NO production by (60-70%) as compared to LPS. UPLC-ESI-MS analysis of the butanol extract in the negative-ion Mode gave compounds such as Gallic acid, while in the positive-ion mode of the butanol extract contains metabolites like syringic acid. Analysis of the Ethyl acetate extract in the negative-ion Mode was 2,3-Dihydroxybenzoic acid and others. Analysis of the ethyl acetate extract in the positive-ion Mode revealed some natural compounds within gallic acid methyl ester. However, these bioactive compounds are responsible for promising inhibition of NO production, antimicrobial and antibacterial activities.

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