Assessment of Urinary 8-Hydroxy-2-Deoxyguanosine as Oxidative DNA Damage for Diabetic Nephropathy

Zaki, Moushira; Abdallah, Hanaa Reyad; Orban, Hisham; Shahba, Mohamed A.; Wafy, Wafaa; Esmail, Maha M; Girgiss, Mina Wassef; Youness, Eman

doi:10.21608/ejchem.2024.256342.8999

Assessment of Urinary 8-Hydroxy-2-Deoxyguanosine as Oxidative DNA Damage for Diabetic Nephropathy

Document Type : Original Article

Authors

¹ Biological Anthropology Department, Medical Research and Clinical Studies Institute–National Research Centre Cairo, Egypt.

² Medical Biochemistry, Departments, Medical Research and Clinical Studies Institute – National Research Centre Cairo, Egypt.

³ Departments of Public Health, Theodor Bilharz Research Institute

⁴ Department of Internal Medicine, Faculty of Medicine, Al-Azher University (for girls), Egypt.

⁵ Medical Department, Medical Research and Clinical Studies Institute, National Research Centre Cairo, Egypt.

10.21608/ejchem.2024.256342.8999

Abstract

Diabetic nephropathy (DN) is the main cause of chronic kidney disease, and represents the most common and serious complication of diabetes. 8-hydroxy-2′-deoxyguanosine (8-OHdG) emerged as a marker of DNA damage. Therefore, the study will assess association between urinary 8-OHdG and DN as early biomarker for DN. The study group included 100 volunteers, 50 cases with DN and 50 healthy control subjects matched in age with cases. The glycated hemoglobin (HbA1c), hsCRP, 25 OH vitD3 and urinary 8-OHdG levels were measured. Anthropometric measurements comprised body weight, height, waist circumference (WC) were measured. Significant increase in levels of hemoglobin (HbA1c), urinary 8-OHdG, BMI and WC in DN group compared to control. Significant positive linear relation was observed between WC and urinary 8-OHdG. Moreover, significant decline of vitamin D3 and significant increase of ESR and CRP was observed in DN cases. Oxidative damage had a role in the development of DN and increased levels of 8-Hydroxy-2-Deoxyguanosine is independently associated with WC. Our study found that, both ESR and hs-CRP were increased in DN that may be independent risk factors correlated with the severity of disease.

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