Therapeutic efficacy of Zingiber officinale loaded chitosan nanoparticles in intestinal murine trichinellosis..

The purpose of this research was to compare the impacts ofZingiber officinale extract and its chitosan(CS) nanoparticles(NPs) on the alterations of oxidative stress, histopathological, and parasitological factors in mice infected with Trichinellain comparison to albendazoletreatment as well as groups of controls. The current study included 64 mice, 8 of them were negative controls and 56 of them were infected. The extracts have been administered orally to experimentally infected mice at a dose of 100 mg/kg/day, 50mg/kg/day beginning 24 hours after infection and continued for 14 days on parasite load, intestinal histology, skeletal muscle, heart and oxidative damage. With the exception of the healthy control group, all groups wereinfected orally with T. spiralislarvae at a dose of 200 larvae per mouse. On the 35th day following infection, all groups weresacrificed. The results demonstrated a significant reduction in the mean number of T. spiralisadult worms and larvae count was observed in all treated groups(74.82%, 62.47%, 48.71%, 30.30% & 24.47%)&(71.80%,59.26%, 50.39,37.59% &27.41%) respectively.

The group of mice that received Zingiber officinale NPs exhibited the greatest reduction in mature worm and larvae counts, at 100 and 50 Mg/kg, respectively. For the group treated by Zingiber officinale NPs100 ml/kg, it showed better rank in adult worms and and larvae countcompared to other infected and treated groups and albendazole administered infected group. This was further demonstrated by decreased parasite load, histological changes, and oxidative stress as compared to the control and albendazole-treated infected groups. SOD,GSH concentrations have been investigated in sera and showed that all treatment of infected mice with Zingiberofficinale extract 50 mg/kg and its chitosan nanoparticles, 100 mg/kg considerably improved (P>0.01)all parameter levels in the sera, bringing them back to the original values of the negative control. Finally, we can infer that the usage of Zingiberofficinale NPs may have therapeutic promise in the treatment of trichinellosis.